A large-scale sequencing effort explored microbial life in soils and permafrost of Barrow Environmental Observatory.
Deep DNA sequencing was used to discover microbial functionality in soils and permafrost of the Barrow Environmental Observatory. Through a US Department of Energy Joint Genome Institute Community Sequencing Program grant, the team sequenced soil and permafrost samples collected from different polygon types. Permafrost profiles reached up to 3.5 m in depth. Active layer samples were sequenced in triplicate and two different seasons. This sequencing effort created 1.6 TB of data to analyze microbial functions across topographical features and at different depths to understand and predict future climate trends and threads. The Joint Genome Institute's Integrated Microbial Genomes & Microbiomes system was used to identify microbial populations and genes that drive greenhouse gas fluxes in the tundra.
Citation: Tas, N., B. Dafflon, C. Ulrich, Y. Wu, S. G. Tringe, and J. K. Jansson. 2022. “Metagenomes from Arctic soil microbial communities from the Barrow Environmental Observatory, Utqiaġvik, AK, USA.” Microbiology Resource Announcements 11: 8. https://doi.org/10.1128/mra.00528-22.